详细信息
文献类型:期刊文献
中文题名:三七剪口指纹图谱研究及应用
英文题名:Application of HPLC fingerprint to analysis of rhizome of Panax notoginseng
作者:张敏[1];胡坪[1];罗国安[2,3];王义明[4]
机构:[1]华东理工大学化学与分子工程学院;[2]华东理工大学药学院中药现代化工程中心;[3]清华大学生命科学与医学研究院,北京100084;[4]清华大学生命科学与医学研究院
年份:2007
卷号:38
期号:3
起止页码:442
中文期刊名:中草药
外文期刊名:Chinese Traditional and Herbal Drugs
收录:CSTPCD;;Scopus;北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;
基金:国家高技术研究发展计划(863计划)(2003AA2Z2021)
语种:中文
中文关键词:三七剪口;高效液相色谱;指纹图谱
外文关键词:the rhizome of Panax notoginseng (Burk.). F. H. Chen ; HPLC ; fingerprint
摘要:目的建立三七剪口的HPLC指纹图谱,同时用于不同产地药材的皂苷成分定量。方法采用梯度洗脱法,对三七剪口水提物进行HPLC测定,流动相为水-乙腈,检测波长203nm;记录时间65min。结果运用梯度洗脱的方法能分离三七剪口的各类组分,并可对其中三七皂苷R1、人参皂苷Rg1、Re、Rb1和Rd进行定量。结论本实验建立的HPLC指纹图谱和测定方法可作为三七剪口有效组分的控制手段。
Objective To establish the HPLC fingerprint method of the rhizome of Panax notoginseng and set up a method for content determination of the main ginsenosides in the rhizome of P. notoginseng from various habitats. Methods HPLC Analysis was carried on C18 column with flow phase wateracetonitrile. The detection wavelength was at 203 nm, record time was 65 min. Results The components can be separated well and the ginsenosides Rg1, Re, Rb1, and Rd and notoginsenoside R1 can be determinated using this method. Conelusion The HPLC fingerprint of the rhizome of P. notoginseng and the determination method which developed in this paper are suitable for the quality control of the rhizome of P. notoginseng.
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