文献类型：期刊文献

英文题名：Transposon insertion sequencing analysis reveals conditional essential genes for infection in Pseudomonas plecoglossicida

作者：Ding, Haoyuan[1];Ahat, Peherden[1];Song, Wei[2];Zhang, Yuanxing[3];Choi, Sang Ho[5];Zhang, Yibei[1,3,4];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab Ocean & Polar Fisheries, Minist Agr, Shanghai 200090, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Lab Aquat Anim Dis MARA, Shanghai 200237, Peoples R China;[5]Seoul Natl Univ, Dept Agr Biotechnol, Natl Res Lab Mol Microbiol & Toxicol, Seoul 08826, South Korea

年份：2025

卷号：607

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001496541400001)】；

基金：This work was financially supported by National Key Research and Development Program of China (2022YFE0101200 to QY.W and SH.C) , National Natural Science Foundation of China (32102850 to YB.Z) , Shanghai Agricultural Science and Technology Innovation Project (T2023328 to YB.Z) , and China Agriculture Research System of MOF and MARA (CARS-47) .

语种：英文

外文关键词：Conditional essential genes; Large yellow croaker ( Larimichthys crocea ); MlaE; Pseudomonas plecoglossicida; Transposon insertion sequencing (Tn-seq)

摘要：Pseudomonas plecoglossicida is a critical pathogen that affects cultured large yellow croakers (Larimichthys crocea). While several virulence genes in this bacterium have already been identified, a comprehensive understanding of its pathogenicity is still lacking. To address this gap, we constructed the transposon insertion mutant library for P. plecoglossicida and conducted an in vivo screening of this library for the first time. In this study, we revealed that 251 genes are essential, while 145 additional genes are conditionally essential during the infection process. Furthermore, we proposed that the absence of the mlaE (Delta mlaE) resulted in an attenuated phenotype, emphasizing the ability of Delta mlaE to be a live attenuated vaccine due to its reduced ability to survive under host's antimicrobial defenses. This study employed a powerful tool for functional genomic analysis in P. plecoglossicida, and provided a more holistic view of its pathogenicity. By gaining more insights into the virulence mechanisms of P. plecoglossicida, we can potentially develop more effective therapeutic strategies against its infections.