文献类型：期刊文献

中文题名：聚乙二醇改性壳聚糖固定化L-天门冬酰胺酶的工艺研究

英文题名：Experimental Study on Preparation and Properties of Immobilized L-Asparaginase on Chitosan Modified by Poly(ethylene glycol)

作者：霍红光[1];方波[1];江体乾[1]

机构：[1]华东理工大学化学工程研究所,上海200237

年份：2005

卷号：31

期号：1

起止页码：13

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2005149026091);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：聚乙二醇改性壳聚糖;L-天门冬酰胺酶;固定化酶

外文关键词：chitosan modified by poly(ethylene glycol); L-asparaginase; immobilized enzyme

摘要：采用交联法制备了聚乙二醇改性壳聚糖(PEG-CS)载体,并将其用于固定化L-天门冬酰胺酶。研究了聚乙二醇浓度、交联剂戊二醛浓度、活化剂甲醛浓度、酶溶液浓度等因素对PEG-CS固定化L-天门冬酰胺酶活力的影响。结果表明:在适当的条件下,PEG-CS固定化L-天门冬酰胺酶的活力可达20～40U/g,并且PEG-CS固定化L-天门冬酰胺酶具有良好的生化性质。
PEG-CS (chitosan modified by polyethylene glycol), a kind of carrier, was prepared and applied to immobilize L-asparaginase. The preparation and properties of immobilized L-asparaginase on PEG-CS were studied. The effects of reaction process, immobilization time, and concentrations of PEG, cross-linking agent, activation reagent and enzyme solution on the immobilized L-asparaginase were investigated. The results show that, the activity of the immobilized L-asparaginase can reach as high as 20-40 U/g under proper conditions for L-asparaginase-immobilization, and the immobilized L-asparaginase possesses good biochemical properties.