文献类型：期刊文献

中文题名：罗汉菜籽总黄酮的提取、纯化与体外抗氧化活性研究

英文题名：Isolation,Purification and Antioxidant-Activity-Determination in vitro of Flavonoids from Thlaspi Arvense Linn Seeds

作者：朱明明[1];高天怡[1];徐志珍[1];周佳祺[1];李俊雯[1];陈珏[2];张文清[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]上海市嘉定区农业技术推广服务中心,上海201800

年份：2019

卷号：40

期号：8

起止页码：75

中文期刊名：食品研究与开发

外文期刊名：Food Research and Development

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：上海市科委科研计划项目(13391900500)

语种：中文

中文关键词：罗汉菜籽;黄酮;提取优化;纯化;体外抗氧化

外文关键词：Thlaspi Arvense Linn seeds;flavonoids;isolation;purification;antioxidant activity in vitro

摘要：采用正交试验法优化罗汉菜籽总黄酮提取工艺,最终确定的最佳提取条件为:乙醇体积浓度60%,料液比1∶30(g/mL),提取时间为75 min,提取次数2次,所得总黄酮粗提物含量为10.3 mg/g。采用制备色谱对总黄酮粗提物进行纯化,将提取含量由10.3 mg/g提升至563.8 mg/g。对总黄酮粗提物和纯化物进行体外抗氧化活性研究,包括对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐阳离子(ABTS+)自由基清除能力的测定及铁还原能力(ferric ion reducing antioxidant power,FRAP)的测定,结果表明粗提物和纯化物都有较好的抗氧化活性,且纯化后效果更显著。
Orthogonal experimental design was used to extract flavonoids from Thlaspi Arvense Linn seeds,and the optimized extraction conditions were as follows:using 60%ethanol as solvent,the ratio of material to liquid was 1∶30(g/mL),extraction time was 75 min for twice.And under this extraction condition,the concentration of flavonoids was 10.3 mg/g.Preparative chromatography was also applied to purify flavonoids,and the purity raised to 563.8 mg/g from 10.3 mg/g.Different free radical antioxidant experiments,including DPPH,ABTS+and FRAP,were applied to determine the antioxidant effect of crude and purified flavonoids.The results showed that both crude and purified flavonoids exhibited high antioxidant activity,and purified flavonoids had a better effect than crude flavonoids.