文献类型：期刊文献

中文题名：载rhBMP-2聚乳酸/磷酸钙支架在兔脊柱融合中的应用

英文题名：A Polylactic Acid/Calcium Phosphate Scaffold with rhBMP-2 for Spinal Fusion in Rabbit

作者：王萌[1];张爽[2];王靖[2];曹雪华[1];岳述荣[1];刘昌胜[2]

机构：[1]上海瑞邦生物材料有限公司,上海201707;[2]华东理工大学材料科学与工程学院,上海200237

年份：2023

卷号：49

期号：6

起止页码：787

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：上海市科技创新行动计划(19441916400)。

语种：中文

中文关键词：骨修复材料;聚乳酸;磷酸三钙;骨形态发生蛋白;脊柱融合

外文关键词：bone repair material;polylactide acid;tricalcium phosphate;bone morphogenetic protein;spinal fusion

摘要：脊柱融合术是治疗脊柱退变性疾病的常用方法,提高植骨材料的成骨活性对提高脊柱融合效果具有重要意义。重组人骨形态发生蛋白-2(rhBMP-2)是一种促进新骨生成的生长因子,能够快速诱导骨修复。将rhBMP-2通过物理吸附负载于聚乳酸/磷酸钙多孔支架表面,再将表面涂覆羟丙甲基纤维素(HPMC)涂层,得到HPMC涂层改性的载rhBMP-2聚乳酸/磷酸钙支架。rhBMP-2的负载使支架具有诱导成骨活性,而HPMC涂层能够使rhBMP-2的释放减缓,有利于促进新骨生成。将该支架植入兔脊柱84 d后发现脊柱椎体间生成了连续的骨组织,达到了骨性融合,因此HPMC改性的载rhBMP-2聚乳酸/磷酸钙支架是一种可用于脊柱融合植骨的理想材料。
Spinal fusion is a commonly used method for the treatment of spine degenerative diseases.To achieve spine fusion successfully,the osteogenic activity of bone-graft materials needs to be high enough to promote bony fusion.Recombinant human bone morphogenetic protein-2(rhBMP-2)is a growth factor that promotes new bone formation and induces bone repair rapidly.In this study,a porous polylactic acid/calcium phosphate scaffold was prepared,to which rhBMP-2 was loaded by physical adsorption to make the scaffold osteoinductive.Furthermore,the rhBMP-2-loaded scaffold was coated with hydroxypropyl methylcellulose(HPMC)for the sustained release of rhBMP-2.The HPMC coated scaffold bearing rhBMP-2 was implanted in rabbit spines.We observed a continuous bone tissue generation at 12 weeks post-operation,and the spines were bony fused.Our results suggest the promise of using the HPMC-coated polylactic acid/calcium phosphate scaffold bearing rhBMP-2 as a bone-repairing material for spinal fusion.