文献类型：期刊文献

英文题名：Methanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days

作者：Wang, Li-Ying[1,2];Li, Wei[1,2];Mbadinga, Serge Maurice[1,2];Liu, Jin-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2012

卷号：29

期号：8

起止页码：716

外文期刊名：GEOMICROBIOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000306460900004)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

语种：英文

外文关键词：microbial community; methanogenesis; n-alkanes; oily sludge

摘要：Methanogenic microbial community is responsive to the availability of hydrocarbons and such information is critical for the assessment of hydrocarbon degradation in remediation and also in biologically enhanced recovery of energy from non-producing oil reserves. In this study, methanogenic enrichment cultures from oily sludge amended with n-alkanes (C-15-C-20) showed a development of active methanogenic alkanes-degrading consortium for over a total of 1000 days of incubation at 37 degrees C. Total genomic DNAs were extracted from three types of samples, the original oily sludge (OS), the sludge after incubation for 500 days under methanogenic condition without any external carbon addition (EC), and the enrichment culture from the EC amended with n-alkanes (ET) incubated for another 500 days. The phylogenetic diversities of microbial communities of the three samples were analyzed by PCR amplification of partial 16S rRNA genes. The catabolic genes encoding benzylsuccinate synthase (bssA) and alkylsuccinate synthase (assA) were also examined by PCR amplification. These results provide important evidence in that microbial populations in an oily sludge shifted from methanogenic aromatic compounds degrading communities to potential methanogenic alkane-degrading communities when the enrichment was supplemented with n-alkanes and incubated under anaerobic conditions.