文献类型：期刊文献

英文题名：Preliminary study of fermentation conditions for producing DNA vaccine by recombinant escherichia coli

作者：Ke, Wei; Xia, Jie; Lu, Bing; Xu, Dian-Sheng

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2006

卷号：32

期号：1

起止页码：38

外文期刊名：Huadong Ligong Daxue Xuebao /Journal of East China University of Science and Technology

收录：EI(收录号：2006089715999)

语种：英文

摘要：The fermentation conditions of Escherichia coli DH5α for the production of tuberculosis DNA vaccine were studied. The method of uniform design being applied to the design of culture medium, it was found that the productivity of the plasmid DNA per cell was optimal when the ratio of trypton and yeast extract was 1:1.2 and the quantity of phosphate added was adequate. Compared with the fed-batch operation with constant glucose feed rate, the fed-batch operation with exponential glucose feed rate has an increased yield of plasmid DNA per cell while the yield of cell per gram of glucose remains the same. The stepwise increase of the culture temperature from 37°C to 42°C in the stationary phase enhanced the productivity of plasmid DNA to 75 mg/L while the biomass concentration was the same in the process with constant temperature of 37°C.