文献类型：期刊文献

中文题名：林可霉素生物合成培养基的优化

英文题名：Optimization of biosynthesis medium for lincomycin production

作者：周晓芳[1];叶蕊芳[1];储成亮[1];马立新[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2011

卷号：41

期号：2

起止页码：21

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：林可霉素;Plackett—Burmani面分析;基优化

外文关键词：lincomycin; Plackett-Burman; response surface methodology; medium optimization

摘要：以花生粉和棉籽蛋白粉取代了原培养基中的黄豆饼粉,采用响应面法对林可霉素产生菌的发酵培养基进行了优化。首先通过单因素试验及正交实验确定替代氮源及其浓度,采用Plack-ett-Burman实验分析各因素的主效应,选出对响应值影响较大的3个因素,即花生粉、K_2HPO_4和玉米浆。对这些因素做爬坡实验,确定三个重要因素的中心点浓度,并运用响应面分析法确定了这3个重要因素的最优水平,分别为熟花生粉18g/L、K_2HPO_40.4g/L、玉米浆1.2g/L。采用优化后的培养基进行摇瓶发酵,林可霉素摇瓶生物效价为4.268g/L,比优化前提高了12%～17%。最后分别比较优化前后培养基中金属离子和氨基酸含量的差别,发现金属离子和氨基酸对林可霉素生物合成有较大影响。
The soybean flour was replaced by cottonseed protein and peanut meal as main nitrogen sources for lincomycin production. Single factor experiments and orthogonal test were carried out to determine the alternative nitrogen source and concentration. The response surface methodology was used to optimize the biosynthesis medium of Streptomyces linconinensis. First, peanut meal, KH2PO4 and corn steep liquor were found to be the important factors by Plackett-Burman design. Then center concentration of the three important factors was determined by the steepest ascent search. And the optimal concentration of the three important factors was defined by response surface methodology. The results were the following： 18 g/L peanut meal, 0.4 g/L KH2PO4 and 1.2 g/L corn steep liquor. The average potency of lincomycin could reach 4. 268 g/L by using the optimal medium, which was increased by 12% -17% compared with that of the original medium. Finally, metal ions and amino acid had good effects on lincomycin production by comprising differences between original culture and optimal one.