文献类型：期刊文献

英文题名：Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli

作者：Du, Fu-Liang[1]; Yu, Hui-Lei[1]; Xu, Jian-He[1]; Li, Chun-Xiu[1]

机构：[1] Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2014

卷号：1

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513087720)

语种：英文

外文关键词：Biochemistry - Biosynthesis - Flavor compounds - Gene expression - Monoterpenes

摘要：Background: Limonene is an important monoterpene used as a chemical commodity and precursor for producing biofuels, flavor and medicinal compounds. Results: In this paper, we engineered Escherichia coli by embedding two exogenous genes encoding a limonene synthase (LS) and a geranyl diphosphate synthase (GPPS) for production of limonene. Out of 12 E. coli strains transformed with various plasmids, the best one with p15T7-ls-gpps produced limonene with a titer of 4.87 mg/L. In order to enhance the limonene production, two rate-limiting enzymes in the endogenous MEP pathway of E. coli, 1-deoxy-xylulose-5-phosphate synthase (DXS) and isopentenyl diphosphate isomerase (IDI), were overexpressed consecutively on vector pET21a+, resulting in a production of 17.4 mglimonene/L at 48 h. Conclusions: After the preliminary optimization of the medium in a two-phase culture system composed of n-hex-adecane (1/50, Vorg/Vaq), the final production of limonene was raised up to 35.8 mg/L, representing approximately a 7-fold improvement compared to the initial titer. ? 2014 Du et al.