文献类型：期刊文献

英文题名：Enhanced Steroid Metabolites Production by Resting Cell Phytosterol Bioconversion

作者：Gao, X. -Q.[1];Feng, J. -X.[1];Wang, X. -D.[1];Hua, Q.[1];Wei, D. -Z.[1]

机构：[1]E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：29

期号：4

起止页码：567

外文期刊名：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY

收录：;EI(收录号：20170803379433);WOS:【SCI-EXPANDED(收录号:WOS:000368438700009)】；

基金：This study was supported by the National Natural Science Foundation of China (General Program, No. 21276083).

语种：英文

外文关键词：phytosterol; steroid metabolites; resting cell bioconversion; hydroxypropyl-beta-cyclodextrin; optimization

摘要：The steroid metabolites 9-hydroxy-androstenedione (9-OH-AD), androstadienedione (ADD) and androstenedione (AD) are important steroidal pharmaceuticals. In order to raise the production of steroid metabolites, an efficient resting cell phytosterol biocon-version process was developed to produce 9-OH-AD in the presence of hydroxypropyl-beta-cyclodextrin (HP-beta-CD). Cell growth medium containing phytosterol as an inducer positively improved cell activity. Under aerobic conditions, bioconversion proceeded at 70 g L-1 phytosterol in the presence of HP-beta-CD (the optimized molar ratio of - HP-beta-CD/phytosterol was 1: 1) with 30 g L-1 resting Mycobacterium neoaurum NwIB-yV cells (cell dry mass) in a 5-L bioreactor, where 9-OH-AD production and space-time yield reached 36.4 g L-1 and 9.1 g L-1 d(-1), respectively. The recycling of cells and HP-beta-CD enables cost-saving and industrial applications. This bioprocess was also applied for the production of ADD and AD. The production of these steroid metabolites was much higher than that reported in previous studies.