文献类型：期刊文献

中文题名：香菇菌糠高温高压-复合酶降解工艺优化及提取物的生物活性

英文题名：Extraction Optimization of High Temperature and Pressure Complex Enzyme Degradation of Spent Lentinula edodes Cultivation Substrate and Biological Activities of the Extract

作者：马冰清[1];张忠[2];吴迪[2];范立强[1];张春月[1];周峰[2];杨焱[2];赵黎明[1]

机构：[1]华东理工大学生物工程学院,上海200237;[2]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,农业农村部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海201403

年份：2021

卷号：28

期号：4

起止页码：27

中文期刊名：食用菌学报

外文期刊名：Acta Edulis Fungi

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：上海市科技兴农项目(2021-02-08-00-12-F00753);国家重点研发计划课题(2019YFD0901805);上海市曙光计划项目(15SG28);高等学校学科创新引智计划项目(B18022)。

语种：中文

中文关键词：香菇菌糠;高温高压-复合酶;抗炎活性;抗氧化活性

外文关键词：Shiitake mushroom cultivation residue;high temperature and pressure-complex enzyme;antiinflammatory activity;antioxidant activity

摘要：采用单因素和响应面实验优化了高温高压-复合酶法处理香菇(Lentinula edodes)废弃菌糠的条件:料液比1∶20(g∶mL),最佳复合酶配比为纤维素酶∶木聚糖酶∶β-葡聚糖酶=3∶2∶1,总加酶量660U·g^(-1),pH 5.20,温度45℃,酶解时间6h,在此条件下总糖提取率为13.4%,提取物得率为22.4%。体外抗氧化实验表明,高温高压-复合酶法降解香菇菌糠得到的提取物(LR-UE)较水提法和高温高压法得到的提取物(LR-W和LR-U)具有更强的DPPH和ABTS自由基清除能力。体外细胞实验表明,LR-UE能提高脂多糖(LPS)刺激巨噬细胞RAW 264.7释放NO的抑制率,表现出良好的体外抗炎活性。研究结果为香菇菌糠的高值化利用提供了新思路。
The rapid development of Lentinula edodes cultivation in China has brought about a substantial increase in cultivation residue waste which is calling for sustainable and eco-friendly recycling.In this study,single factor and response surface experiments were carried out to optimize the conditions for processing L.edodes cultivation residue by high temperature and pressure-complex enzyme.The optimal conditions were as follows:complex enzyme composed of cellulase,xylanase andβ-glucanase at 3∶2∶1,complex enzyme quantity of 660U·g^(-1),material-liquid ratio of 1∶20(g∶mL),pH 5.20,temperature at 45℃,enzymolysis time for 6h.Using these conditions,the overall extraction rate was 22.4%,and the total sugar extraction rate was 13.4%.Antioxidant experiments in vitro showed that the extract obtained through the high temperature and pressure complex enzyme(LR-UE)had stronger activities on scavenging 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and 2,2′-diazo-bis-3-ethylbenzothiazolin-6-sulfonic acid(ABTS)than the extracts obtained by water extraction(LR-W)and high temperature and pressure(LR-U).LR-UE also increased the inhibition rate of NO release from RAW264.7macrophage cells upon lipopolysaccharide(LPS)stimulation,showing good anti-inflammatory activity in vitro.These results provided a new idea for highvalue utilization of Lentinula edodes cultivation residue.