文献类型：期刊文献

中文题名：Construction and optimization of a biocatalytic route for the synthesis of neomenthylamine from menthone

作者：Hui-Jue Zhu[1];Jiang Pan[1];Chun-Xiu Li[1];Fei-Fei Chen[1];Jian-He Xu[1]

机构：[1]Laboratory of Biocatalysis and Synthetic Biotechnology,State Key Laboratory of Bioreactor Engineering,Shanghai Collaborative Innovation Centre for Biomanufacturing,College of Biotechnology,East China University of Science and Technology,Shanghai 200237,People’s Republic of China.

年份：2023

卷号：10

期号：1

起止页码：347

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：financially supported by the National Key Research and Development Program of China(2019YFA0905000 and 2021YFA0911400);the National Natural Science Foundation of China(21871085 and 31971380);the Fundamental Research Funds for the Central Universities(222201714026).

语种：英文

中文关键词：Biocatalysis;(+)-Neomenthylamine;Transaminase;Asymmetric synthesis;(+)-N-Boc-Neomenthylamine

摘要：(+)-Neomenthylamine is an important industrial precursor used to synthesize high value-added chemicals.Here,we report a novel biocatalytic route to synthesize(+)-neomenthylamine by amination of readily available(?)-menthone substrate usingω-transaminase.By screening a panel ofω-transaminases,anω-transaminase from Vibrio fluvialis JS17 was identified with considerable amination activity to(?)-menthone,and then characterization of enzymatic properties was conducted for the enzyme.Under optimized conditions,10 mM(?)-menthone was transformed in a mild aqueous phase with 4.7 mM product yielded in 24 h.The biocatalytic route using inexpensive starting materials(ketone substrate and amino donor)and mild reaction conditions represents an easy and green approach for(+)-neomenthylamine synthesis.This method underscores the potential of biocatalysts in the synthesis of unnatural terpenoid amine derivatives.