文献类型：期刊文献

英文题名：Highly stretchable HA/SA hydrogels for tissue engineering

作者：Zhu, Chengcheng[1];Yang, Rui[1];Hua, Xiaobin[1];Chen, Hong[1];Xu, Jumei[1];Wu, Rile[2];Cen, Lian[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, Dept Prod Engn, State Key Lab Bioreactor Engn,Sch Chem Engn, Shanghai, Peoples R China;[2]Inner Mongolia Peoples Hosp, Dept Neurosurg, Hohhot, Peoples R China

年份：2018

卷号：29

期号：5

起止页码：543

外文期刊名：JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

收录：;EI(收录号：20180504685554);WOS:【SCI-EXPANDED(收录号:WOS:000424943700006)】；

基金：This work was supported by the 'Open Funding Project of the State Key Laboratory of Bioreactor EngineerinG'; National '973' Project Foundation [grant number 2010CB944804]; National Natural Science Foundation of China [grant number 21676083].

语种：英文

外文关键词：Hydrogel; stretch; hyaluronic acid; drug release; regeneration

摘要：A highly stretchable hyaluronic acid (HA)/sodium alginate (SA) hydrogel was developed in this study based on an interpenetrating polymer network. HA/SA hydrogels were prepared by mixing two polysaccharides followed by covalent crosslinking via epoxy groups on HA molecules and ionic crosslinking via divalent ions on SA chains sequentially. The effect of HA/SA ratio on the pore size and distribution, swelling ratio, elongation and rheological properties as well as protein loading and release properties of HA/SA hydrogels was explored. Moreover, a surface modification method, layer-by-layer (LBL) assembly technique, was applied to modify the hydrogel to evaluate the hydrogel's tenability in varying biological performance. It was then shown that the hydrogels had the pore sizes ranging from 100 to 50m. With the increase in SA content of the resulting hydrogels, the pore size, swelling ratio, and storage modulus (G) and loss modulus (G) of the hydrogel all decreased, whereas the in vitro bulk weight loss was fastened. Moreover, elongation at break (EB) value increased first, reached a peak value and then decreased, that is HA8/SA1 (HA:SA=8:1) had the highest EB value of 417%. This hydrogel could retain 33.2% of the pre-loaded protein even after 72h, which could be further attenuated when LBL was used to shell the hydrogel. The growth of fibroblasts on HA8/SA1 hydrogel gave preliminary assessment on its suitability as a cellular carrier, while the LBL modified HA8/SA1 hydrogel also favored the anchoring of keratinocytes, further enhancing its cell carrier role for tissue regeneration, especially skin engineering.