文献类型：期刊文献

中文题名：甜菜碱增强延长盐单胞菌高盐适应性的代谢机理解析

英文题名：Analysis of the Metabolic Mechanism for the Enhancement of High Salt Acclimatization of Halomonas elongata Mediated by Betaine

作者：刘昊[1];张悦[1];居润霖[1];银肖剑[1];余君雄[1];王泽建[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：2025

卷号：51

期号：6

起止页码：774

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：国家重大专项项目(2020YFA096800)。

语种：中文

中文关键词：延长盐单胞菌;高盐胁迫;甜菜碱;代谢动力学建模;代谢物分析

外文关键词：Halomonas elongata;salt stress;betaine;metabolic kinetics modeling;metabolite analysis

摘要：本研究针对延长盐单胞菌(Halomonas elongata)在高盐胁迫下生长受抑制的瓶颈问题,系统筛选了可缓解盐抑制效应的保护因子,结果表明甜菜碱的添加能够使菌体生物量提高18%,显著优于其他渗透调节剂。代谢动力学建模分析表明甜菜碱添加后菌体的最大比生长速率(μ_(max))显著增加42%、呼吸强度提高118%。碳分布统计结果表明甜菜碱添加后依克多因合成在延滞期明显减弱,碳代谢向细胞菌体生长迁移。胞内关键代谢物分析表明,甜菜碱的添加显著降低丙氨酸、缬氨酸和亮氨酸水平,揭示其可能通过增强三羧酸(TCA)循环代谢通量以提升盐适应能力。该研究为构建高盐环境下嗜盐菌高效表达体系提供了理论依据与工艺优化策略。
In this study,to address the bottleneck problem of growth inhibition under high salt stress in Halomonas elongata,we conducted a systematic selection of protective factors that could relieve the effect of salt stress inhibition.The results showed that the addition of betaine was able to increase the biomass by 18%,which was significantly better than that achieved with other osmotic regulators.Furthermore,metabolic kinetic modeling analysis showed that the maximum specific growth rate(μ_(max))of the bacterium significantly increased by 42%after the betaine addition,and the respiratory intensity increased by 118%.Carbon distribution statistics showed that ectoine synthesis was significantly weakened during the delayed phase after betaine addition,and carbon metabolism was shifted toward growth of the cells.Finally,the analysis of intracellular key metabolites showed that the addition of betaine significantly reduced the levels of alanine,valine and leucine,revealing that it may enhance the salt adaptation capacity of Halomonas elongata by strengthening the metabolic flux of the tricarboxylic acid(TCA)cycle.This study provides a theoretical basis and process optimization strategy for the construction of an efficient expression system for halophilic bacteria in high salt environment.