文献类型：期刊文献

英文题名：ELECTRICAL NOISES REDUCTION IN NANOPORES EXPERIMENTS BASED ON CONSENSUS FILTER

作者：Yan, Bingyong[1];Cui, Haixu[1];Zhou, Jiale[2];Wang, Huifeng[1]

机构：[1]East China Univ Sci & Technol, Sch Informat Sci & Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：43

期号：7

起止页码：837

外文期刊名：QUIMICA NOVA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000561987100001)】；

基金：This work was supported by National Natural Science Foundation of China (NO.51407078 and 61773165).

语种：英文

外文关键词：nanopores; consensus filter; DNA sequencing; bessel filter

摘要：Nanopore has developed into an indispensable tool for single molecules experiments, where useful information about the characteristics of DNA/RNA can be obtained from the flowing of ionic current elicited by individual molecules as they traverse a single nanometer-scale aperture. However, due to the relatively high background current noise and low measurement bandwidth, it is difficult to distinguish the molecule-specific signals from the intrinsic noises of the nanopore recording systems, which impeded its application in DNA sequencing. In this paper, we present a novel consensus filter-based noise reduction approach, which considerably improves the accuracy of the noise-filtered current traces and the signal-to-noise ratios (SNRs). The new approach is compared to the conventional Bessel filters and validated by analyzing simulated representative translocation data as well as alpha-Hemolysin (alpha-HL) nanopore experimental data. It is demonstrated that the high frequency noise generated by the voltage noise of the amplifier and Analog-to-Digital converter circuits (ADC) can be significantly reduced and some hidden short-lived translocation events can be recovered with satisfying accuracy beyond the conventional limitations of the Bessel filters.