文献类型：期刊文献

中文题名：鸡红细胞Cu,Zn-SOD的纯化及部分性质研究

英文题名：Study on Purification and Properties of Superoxide Dismutase from Erythrocytes of Chicken

作者：张兰杰[1];候冬岩[1];辛广[1];袁勤生[2]

机构：[1]鞍山师范学院化学系,辽宁鞍山114007;[2]华东理工大学生命科学学院,上海200237

年份：2008

卷号：29

期号：2

起止页码：266

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：鸡红细胞;铜锌超氧化物歧化酶;纯化;性质

外文关键词：erythrocytes of chicken; copper and zinc superoxide dismutase; purification; property

摘要：目的:从鸡红细胞分离铜锌超氧化物歧化酶(Cu,Zn-SOD),并对其部分性质进行研究。方法:用氯仿-乙醇除杂蛋白、丙酮沉淀、热变性、DEAE-32柱色谱等方法,对鸡红细胞Cu,Zn-SOD进行分离纯化。结果:得到Cu,Zn-SOD干粉5.9mg,比活10196.27U/mg蛋白,活力回收70.43%,纯化倍数为125.82。理化性质分析表明:该酶的最大紫外吸收波长264nm,相对分子量约为31000D,相对亚基分子量约为16500D,pH在6～9范围内稳定性很好,在40～60℃内保温30min酶活基本不变,2mmol/LSDS对此酶没有明显的抑制,该酶对H2O2和KCN敏感。结论:鸡红细胞含有丰富的Cu,Zn-SOD。
Objective： To purify copper/zinc superoxide dismutase （Cu, Zn-SOD） from erythrocytes of chicken and to study some properties of purified Cu, Zn-SOD. Methods： Cu, Zn-SOD has been purified from erythrocytes of chicken by chloroform- ethanol fractionation, acetone precipitation, thermal denaturalization and I）EAE-32 chromatography. Results： 5.9 mg of dry powder enzyme was obtained, the purified specific activity of the enzyme was 10196.27 U/mg protein, the yield was 70.46%, and its purification time was 125.82. The investigation of physiochemical properties of the SOD showed that the ultraviolet absorption peak of the enzyme was found to be at 264 nm, which has two identical subunit, the molecular weight about 31000 D and the molecular weigh of subunit about 16500 D. The enzyme was found to have good stability at the pH range from 6 to 9 and good thermal stability at the 40℃ to 60 ℃ range over 30 mimutes incubation period. The Cu, Zn-SOD was not obviously inhibited by 2 mol/L SDS, but obviously inhibited by H2O2 and KCN. Conclusion： Cu, Zu-SOD is rich in the erythrocytes of chicken.