文献类型：期刊文献

英文题名：Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA

作者：Zuo, Fangting[1,2];Jiang, Li[3];Su, Ni[1,2];Zhang, Yaqiang[1,2];Bao, Bingkun[3];Wang, Limei[1,2];Shi, Yajie[1];Yang, Huimin[1,2];Huang, Xinyi[3];Li, Ruilong[1];Zeng, Qingmei[3];Chen, Zhengda[1,2];Lin, Qiuning[3];Zhuang, Yingping[4];Zhao, Yuzheng[1,2,5];Chen, Xianjun[1,2,5];Zhu, Linyong[1,3];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Sch Bioengn, Shanghai, Peoples R China;[5]Chinese Acad Med Sci, Res Unit New Tech L Cell Metab Imaging, Beijing, Peoples R China

年份：2024

卷号：20

期号：10

外文期刊名：NATURE CHEMICAL BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001230106900003)】；

基金：This research was supported by the National Key Research and Development Program of China (grant nos. 2022YFC3400100 to Y.Y. and X.C., 2019YFA0110500 to L.Z. and 2019YFA0904800 to Y.Y. and L.Z.), NSFC (grant nos. 32121005, 92357308, 32150028 and 21937004 to Y.Y.; 21907029 and 21877037 to L.Z.; 32250009 to X.C. and 32001051 to N.S.), STI2030-Major Projects (grant nos. 2021ZD0202200 and 2021ZD0202203 to X.C.), the Shanghai Municipal Education Commission (grant no. 2021 Sci & Tech 03-28 to Y.Y. and X.C.), the Shanghai Science and Technology Commission (23J21900400 to X.C.), the State Key Laboratory of Bioreactor Engineering (to Y.Y. and X.C.) and the Fundamental Research Funds for the Central Universities (to Y.Y. and X.C.).

语种：英文

摘要：Fluorescent RNAs (FRs) provide an attractive approach to visualizing RNAs in live cells. Although the color palette of FRs has been greatly expanded recently, a green FR with high cellular brightness and photostability is still highly desired. Here we develop a fluorogenic RNA aptamer, termed Okra, that can bind and activate the fluorophore ligand ACE to emit bright green fluorescence. Okra has an order of magnitude enhanced cellular brightness than currently available green FRs, allowing the robust imaging of messenger RNA in both live bacterial and mammalian cells. We further demonstrate the usefulness of Okra for time-resolved measurements of ACTB mRNA trafficking to stress granules, as well as live-cell dual-color superresolution imaging of RNA in combination with Pepper620, revealing nonuniform and distinct distributions of different RNAs throughout the granules. The favorable properties of Okra make it a versatile tool for the study of RNA dynamics and subcellular localization. Zuo et al. developed a highly bright and stable green fluorescent RNA for robust imaging of the dynamics of messenger RNA in living cells, enabling visualization of nonuniform and distinct distributions of different RNAs throughout stress granules.