文献类型：期刊文献

英文题名：Ultrasound-Driven Cu-Nanobots for Tumor Deep Penetration and Induction of Cuproptosis in Prostate Cancer Therapy

作者：Xu, Jiaxi[1];Lv, Mingchen[1];Hu, Wei[1];Chen, Ran[1];Liu, Shangpeng[1];Sun, Min[2,3];Du, Jianzhong[1,2,3];Fan, Zhen[1,2]

机构：[1]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, Shanghai 201804, Peoples R China;[2]Tongji Univ, Shanghai Peoples Hosp 4,Sch Med, Clin Res Ctr Anesthesiol & Perioperat Med,Translat, Dept Gynaecol & Obstet,Shanghai Key Lab Anesthesio, Shanghai 200434, Peoples R China;[3]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：17

期号：34

起止页码：48017

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20253619092267);WOS:【SCI-EXPANDED(收录号:WOS:001550176200001)】；

基金：This research was supported by National Natural Science Foundation of China (52222306, 22335005, 22475154, and 22305177), international scientific collaboration fund of Science and Technology Commission of Shanghai Municipality (23520710900), Innovation Program of Shanghai Municipal Education Commission (2023ZKZD28), and Shanghai Rising-Star Program (Sailing, 23YF1433000). Video Spot Tracker was developed by CISMM at University of North Carolina at Chapel Hill, supported by the NIBIB of the National Institutes of Health (NIH 5-P41-RR02170).

语种：英文

外文关键词：nanobots; ultrasound; penetration; cuproptosis; prostate cancer

摘要：Cuproptosis has attracted considerable attention in cancer therapy due to its unique ability to circumvent drug resistance, while the in vivo application is hindered by systemic toxicity, rapid clearance, and limited tumor tissue penetration. Widely recognized for its noninvasive application, ultrasound has emerged as a promising method for enhancing drug tumor penetration. In this study, we developed cuproptosis-oriented Nanobots to achieve deep penetration into prostate tumors under ultrasound irradiation. Asymmetric Cu-Nanobots (similar to 200 nm) were prepared by ultrasound polymerization using hexachlorocyclotriphosphonitrile (HCCP), 4,4 '-sulfonyldiphenol (BPS), and tannic acid (TA) as raw materials, which can efficiently load Cu2+ and trigger Cu2+ release at acidic tumor microenvironments. Under ultrasound stimulation, Cu-Nanobots showed obvious directional motion with a velocity of 19.49 mu m/s continuously. In vitro cell experiments confirmed that Cu-Nanobots can significantly promote the oligomerization of dihydrolipoamide acetyltransferase (DLAT), downregulate iron-sulfur cluster proteins such as ferredoxin 1 (FDX1) and lipoic acid synthase (LIAS), and induce cuproptosis in human prostate cancer cells. In addition, Cu-Nanobots can effectively penetrate into the deep area of solid tumors in an animal model with a satisfactory tumor inhibition rate of similar to 75.75% under sonication. Overall, these ultrasound-driven cuproptosis-oriented Cu-Nanobots provide optimal insights into effective tumor deep penetration and cuproptosis-based treatment.