文献类型：期刊文献

英文题名：Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae

作者：Huang, Yanna[1];Li, Zhimin[1];Shimizu, Kazuyuki[2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Kyushu Inst Technol, Grad Sch Comp Sci & Syst Engn, Dept Biosci & Bioinformat, Iizuka, Fukuoka 8208502, Japan

年份：2012

卷号：103

期号：1

起止页码：351

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298273600048)】；

基金：This study was supported by the the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204, the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, and the National High Technology Research and Development Program of China, No. 2011AA02A203.

语种：英文

外文关键词：3-Hydroxypropionic acid; 1,3-Propanediol; Klebsiella pneumoniae; Aldehyde dehydrogenase

摘要：In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia coli aldH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield reached 24.4 g/L and 0.18 mol/mol glycerol, respectively, and at the same time 1,3-PDO achieved 49.3 g/L with a yield of 0.43 mol/mol in 24 h. The overall yield of 3-HP plus 1,3-PDO was 0.61 mol/mol. Over-expression of the E. coli AldH also reduced the yields of by-products except for lactate. This study demonstrated the possibility of simultaneous production of 3-HP and 1,3-PDO by K. pneumoniae under anaerobic conditions without supply of vitamin B-12. (C) 2011 Elsevier Ltd. All rights reserved.