文献类型：期刊文献

英文题名：Screening and identification of multi-component in Qingkailing injection using combination of liquid chromatography/time-of-flight mass spectrometry and liquid chromatography/ion trap mass spectrometry

作者：Zhang, Hong-Yang; Hu, Ping; Luo, Guo-An; Liang, Qiong-Lin; Wang, Yu-Li; Yan, Shi-Kai; Wang, Yi-Ming

机构：[1]E China Univ Sci & Technol, Sch Chem & Pharmaceut, Mordern Engn Ctr TCM, Shanghai 200237, Peoples R China;[2]Tsinghua Univ, Modern Res Ctr TCM, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

年份：2006

卷号：577

期号：2

起止页码：190

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000240569500006)】；

语种：英文

外文关键词：Qingkailing injection; identification; mass spectrometry; electrospray; time-of-flight; ion trap

摘要：An approach for screening and identification of multi-component in complex traditional Chinese medicine systems with a combinative LC/MS (MSn) technique was described in this paper. The chemical profile of Qingkailing injection, a well-known traditional Chinese formula in China, was studied using the established method as for an application. Benefit from combining the accurate mass measurement of LC/TOF-MS to generate elemental compositions and the complementary multilevel structural information provided by LC/ion trap MSn, 33 components in Qingkailing injection were identified in all. The three isomers of chlorogenic acid, isochlorogenic acid and neochlorogenic acid which are derived from Flos Lonicerae, one of the medicinal materials in Qingkailing, were differentiated by verifying their MS3 fragmentation data. All the components identified were surveyed and classified according to their medicinal materials derivation. This study is expected to provide an effective and reliable pattern for comprehensive and systematic characterization of the complex traditional Chinese medicine systems. (c) 2006 Elsevier B.V. All rights reserved.