文献类型：期刊文献

英文题名：Continuous enzymatic saccharification and its rheology profiling under high solids loading of lignocellulose biomass

作者：Wang, Ya[1];Yang, Hucheng[2];Zhang, Bin[2];Liu, Xiucai[3];Bao, Jie[1,2]

机构：[1]Shihezi Univ, Sch Chem & Chem Engn, Beisi Rd, Xinjiang 832000, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Cathay Biotech Inc, 1690 Cailun Rd,Zhangjiang Hi Tech Pk, Shanghai 201203, Peoples R China

年份：2022

卷号：186

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20223112452813);WOS:【SCI-EXPANDED(收录号:WOS:000891992900003)】；

基金：This research was supported by the National Natural Science Foundation of China (21978083; 31961133006) and Cathay Biotech Inc., Shanghai, China.

语种：英文

外文关键词：Lignocellulose; Continuous enzymatic saccharification; Rheology; Bioreactors; Dry acid pretreatment; High solids loading

摘要：Continuous saccharification of lignocellulose biomass is an important process option for industrial biorefinery practice. Continuous saccharification in industrial operations needs to be performed under high solids loading to obtain a high titer of fermentable sugars for the consequent fermentation or the simultaneous saccharification and fermentation (SSF), which also produces highly viscous hydrolysates, two orders of magnitude greater than the regular fermentation medium. The rheological properties in the continuous saccharification should be characterized for bioreactor design. This study investigated the process efficiency of continuous enzymatic saccharification of two typical lignocellulose feedstocks (corn stover and wheat straw) under high-solids loading. The rheological change was profiled under varying saccharification parameters such as dilution rate, solids loading, and enzyme dosage. The apparent viscosity of the high solids loading hydrolysates was found in the range of 0.11-0.56 Pa.s, which allowed the efficient transportation by the regular pump in vessels and pipelines. The presence of inhibitors generated from dry acid pretreatment showed a negligible effect on the hydrolysis efficiency of the continuous saccharification and provided an environment away from microbial contaminations in the hydrolysates. The study provided important technical support for the process and reactor designs of continuous saccharification of lignocellulose feedstock under high solids loading.