文献类型：期刊文献

英文题名：Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate

作者：Zhou, XS; Zhang, YX

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2002

卷号：24

期号：17

起止页码：1449

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000177576200013)】；

语种：英文

外文关键词：fermentation; Pichia pastoris; proteolytic degradation; recombinant hirudin; specific growth rate

摘要：The effects of the specific growth rate and methanol concentration on the degradation of hirudin produced by recombinant Pichia pastoris were investigated. When a methanol-limited state and the specific growth rate of 0.02 h(-1) were maintained during the fermentation, a minimum degradation of hirudin and a maximum specific hirudin production rate were achieved. By this strategy, the production of intact recombinant hirudin Hir65 reached 0.7 g l(-1) in fed-batch fermentation. Its proportion was 35% to all forms of hirudin.