文献类型：期刊文献

英文题名：Design of genipin-crosslinked microgels from concanavalin A and glucosyloxyethyl acrylated chitosan for glucose-responsive insulin delivery

作者：Yin, Ruixue[1];Wang, Kemin[2];Du, Shuang[3];Chen, Lu[3];Nie, Jun[3];Zhang, Wenjun[1,4]

机构：[1]E China Univ Sci & Technol, Complex & Intelligent Res Ctr, Sch Mech & Power Engn, Shanghai 200237, Peoples R China;[2]Changzhou Univ, Sch Mat Sci & Engn, Changzhou 213164, Peoples R China;[3]Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China;[4]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK S7N 0W0, Canada

年份：2014

卷号：103

期号：1

起止页码：369

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20140417225510);WOS:【SCI-EXPANDED(收录号:WOS:000332812600049)】；

基金：The authors would like to thank the National Natural Science Foundation of China (21304011). A partial support to this research for Dr. Chris Zhang is received from SHRF Phase I project "BioNEMs" (Saskatchewan, Canada).

语种：英文

外文关键词：Glucose-responsive; Microgel; Chitosan derivative; Concanavalin A; Insulin delivery

摘要：Glucose-responsive systems are significant for self-regulated insulin delivery. The aim of this study was to assess the potential of genipin-crosslinked concanavalin A/GEA-chitosan microgels as a glucose-responsive insulin delivery system. A chitosan derivative (GEA-chitosan) was designed in this study as the polymer ligand of concanavalin A (Con A), which not only exhibits a strong affinity to Con A, but also could be directly crosslinked with Con A by genipin, thus avoiding the modification of Con A during an immobilization process. Glucose responsive microgels were fabricated by the reversed-phase emulsion crosslinking method. The in vitro release of insulin indicated that the insulin release was influenced by glucose concentrations, and a desired pulsatile release behavior was detected in response to step-wise glucose challenges for more than eight cycles. The release data were fitted well to an exponential model, without any significant influence of the surface effect. The released insulin was proved to remain active without destruction of the tertiary structure. The analysis of L929 cells viability suggested that these microgels possessed no in vitro cytotoxicity. The obtained genipin crosslinked Con A/GEA-chitosan microgels might be a potential candidate for self-regulated insulin delivery. (C) 2014 Elsevier Ltd. All rights reserved.