文献类型：期刊文献

英文题名：Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase

作者：Pan, Jiang[1];Kong, Xu-Dong[1];Li, Chun-Xiu[1];Ye, Qin[1];Xu, Jian-He[1];Imanaka, Tadayuki[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ritsumeikan Univ, Coll Life Sci, Dept Biotechnol, Shiga 5258577, Japan

年份：2011

卷号：68

期号：3-4

起止页码：256

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287114900006)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 20773038 and 20902023), Ministry of Science and Technology, P.R. China (Nos. 2007AA02Z225, 2009CB724706 and 2009ZX09501-016), China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204) and Shanghai Leading Academic Discipline Project (No. B505).

语种：英文

外文关键词：Crosslinked enzyme coaggregate; Serratia marcescens lipase; Polyethyleneimine; Coaggregation of enzyme trans-3-(4 '-Methoxyphenyl)glycidic acid; methyl ester (MPGM)

摘要：Crosslinking of enzyme aggregates is a promising method for enzyme immobilization. In this work, crosslinked enzyme coaggregates of Serratia marcescens lipase with polyethyleneimine (CLECAs-SML-PEI) were prepared using polyethyleneimine (PEI) as coprecipitant and glutaraldehyde as crosslinking reagent. The crude lipase solution at a low protein concentration (0.1 mg/ml), with PEI at a mass ratio of 3:1 (PEI/protein, w/w), was found to be most adequate for the coprecipitation of SML. After crosslinking of the coaggregate of SML-PEI with 0.2% (w/v) glutaraldehyde under ambient temperature, over 70% of the total lipase activity was recovered. Compared with the free SML, the optimum temperature of the CLECAs-SML-PEI was enhanced from 50 degrees C to 60 degrees C and its thermal stability was also significantly improved. CLECAs-SML-PEI showed excellent operational stability in repeated use in aqueous-toluene biphasic system for asymmetric hydrolysis of trans-3-(4'-methoxyphenyl)glycidic acid methyl ester (MPGM), without significant inactivation after 10 rounds of repeated use. (C) 2010 Elsevier B.V. All rights reserved.