文献类型：期刊文献

英文题名：Edible-plant derived 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone exert dual effects on invigorating triple-negative breast cancer apoptosis

作者：Yu, Tingting[1];Jiang, Yu[1];Guo, Miaomiao[2];Wei, Xing[1];Xin, Xiujuan[1];An, Faliang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Beijing Technol & Business Univ, Key Lab Cosmet, China Natl Light Ind, Beijing, Peoples R China;[3]Marine Biomed Sci & Technol Innovat Platform Linga, Shanghai, Peoples R China

年份：2024

卷号：3

期号：2

起止页码：222

外文期刊名：FOOD BIOENGINEERING

收录：EI(收录号：20242516285545);WOS:【ESCI(收录号:WOS:001328268300003)】；

基金：This work was supported by the National Key R&D Program of China (2023YFA0914102), the National Natural Science Foundation of China (No. 81703388), the General Financial Grant from the China Postdoctoral Science Foundation (2016M601532, 2018T110359), and Talent Program of East China University of Science and Technology, Funding Project of the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：DMC; G(2)/M arrest; MDA-MB-231 cells; PI3K/Akt/mTOR pathway; beta-tubulin binding

摘要：2 ',4 '-Dihydroxy-6 '-methoxy-3 ',5 '-dimethylchalcone (DMC) is a typical and abundant chalcone compound from the buds of Cleistocalyx operculatus, which is well-known for its edible and medicinal qualities. In this study, DMC showed effective cell cycle arrest at G(2)/M on MDA-MB-231 cells, though dual impacts, including the inhibition of microtubule polymerization through binding to beta-tubulin and the stimulation of reactive oxygen species (ROS) production by inhibiting catalase activity. The increased ROS level inhibited PI3K/Akt/mTOR pathway and further suppressed the expression level of Cdc2, Cdc25C, and Cyclin B1, alongside stimulated the expression level of p21 and p27. Above 29.5% MDA-MB-231 cells were arrested at G(2)/M phase, subsequently undergoing apoptosis due to heightened levels of apoptosis-related proteins Bax and caspase 3. In summary, this study demonstrated that DMC concomitantly plays dual roles in apoptotic inducing by inhibiting the ROS consumption and microtubules formation in triple-negative breast cancer cells.