文献类型：期刊文献

中文题名：反胶束液－液萃取牛血清白蛋白的动力学研究I．萃取过程

英文题名：Kinetics of the Solubilization Using Reversed Micelles I.Extraction of Protein

作者：陆强[1];李宽宏[1];施亚钧[1]

机构：[1]华东理工大学化学工程研究所

年份：1994

卷号：20

期号：6

起止页码：723

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

基金：国家教委博士点基金

语种：中文

中文关键词：牛;血清;清蛋白;反胶束萃取;萃取;动力学

外文关键词：BSA;reversed micelles;extraction;kinetics;electrostatics interaction

摘要：以十六烷基三甲基溴化铵－正己醇－正辛烷反胶束溶液为萃取剂，研究了从水溶液中萃取牛血清白蛋白的动力学。在恒界面池中，通过改变搅拌转速、水相ｐＨ值和离子强度、有机相表面活性剂和助溶剂浓度，测定了在多种条件下的表观传质系数，从而判定萃取过程是由水膜扩散和界面过程共同控制。计算了扩散的传质分系数，进而获取了界面传质分系数随水相条件的变化情况，表明萃取的界面传质为一协同过程，静电作用影响到界面变形的程度，使其对传质速率的影响相当强烈。
The kinetics of BSA extraction has been studied with CTAE-hexanol-octanereversed mice11ar solution.In order to determine the overall mass-transfer coefficients,astirred transfer cell with constant interface was operated under varied conditions,such as thestirring speed,the pH value and ionic strength in aqueous phase,and the concentration ofsurfactant and cosolvent.The results showed that the extraction is controlled together byinterfacial process and diffusion through the aqueous film.The individual mass transfer coefficients of diffusion in aqueous phase were calculated,so that a series of individual interfacialcoefficient at various aqueous phase conditions could be obtained.It shows that the interfacial transport of BSA occurs via a cooperative mechanism.The interfacial transfer rate isstrongly affected by the electrostatics in protein/interfacial interaction,which determines theextent of deformation at the interface.