文献类型：会议论文

英文题名：Monitoring constituent concentrations in bioreactions with attenuated total reflection Fourier transform infrared spectroscopy

作者：Ye, Q; Qiu, J; Zhang, SL

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

会议论文集：7th IFAC International Conference on Computer Applications in Biotechnology (CAB7)

会议日期：MAY 31-JUN 04, 1998

会议地点：OSAKA, JAPAN

语种：英文

外文关键词：infrared spectroscopy; monitoring; partial least square algorithm

摘要：Reaction mixtures of enzyme catalyzed hydrolysis of penicillin and sugar concentrations in plant cell culture were monitored with attenuated total reflection Fourier transform infrared spectroscopy (ATR/FT-IR). Partial least square algorithm was used for estimation and measurement errors for penicillin, 6-aminopenicillanic Acid (6-APA), phenylacetic acid (PAA), glucose, fructose, and sucrose were 0.45, 0.53, 0.59, 0.35, 0.22, and 0.11 g/L respectively in the solutions for calibration. Concentration profiles for these compounds were obtained by using ATR/FT-IR in real reaction and cultivation processes. Copyright (C) 1998 IFAC.