文献类型：期刊文献

英文题名：PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris

作者：Wang, Xiaolong[1];Cai, Menghao[1];Shi, Lei[1];Wang, Qi[1];Zhu, Jinxiang[1];Wang, Jinjia[1];Zhou, Mian[1];Zhou, Xiangshan[1];Zhang, Yuanxing[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2016

卷号：38

期号：2

起止页码：291

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20154301433012);WOS:【SCI-EXPANDED(收录号:WOS:000370374300012)】；

基金：This work was supported by Chinese National High Technology Research and Development Program (No. 2014AA093501), National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and Grants of Young and Middle-aged Leading Science and Technology Innovation Talents from Ministry of Science and Technology of China.

语种：英文

外文关键词：AOX1 promoter; Catabolite repression; Glucose; Glycerol; PpNrg1; Pichia pastoris; Transcriptional regulation

摘要：The regulator in glycerol repression of Pichia pastoris AOX1 promoter (P (AOX1) ) is still unclear. A Cys(2)His(2) zinc finger transcriptional repressor PpNrg1 localized to nucleus and participated in the repression of P (AOX1) in P. pastoris in glucose and glycerol. Quantitative real-time PCR revealed that PpNrg1 repressed expression of numerous genes involved in methanol utilization and peroxisome biogenesis in 0.02 % glucose and 1 % (v/v) glycerol. Electrophoretic mobility shift assay and DNase I footprinting assay revealed that PpNrg1 bound to five sites of P (AOX1) , including two binding sites of PpMxr1, which is an indispensable activator of P (AOX1) in P. pastoris. Transcriptional repressor PpNrg1 suppresses P (AOX1) in glucose and glycerol by directly binding to five sites of P (AOX1) , including two binding sites of transcriptional activator PpMxr1.