文献类型：期刊文献

英文题名：The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs

作者：Lv, Chao[1,2];Wu, Xueting[3];Wang, Xia;Su, Juan[1];Zeng, Huawu[1];Zhao, Jing[5];Lin, Shan[2];Liu, Runhui;Li, Honglin[4];Li, Xuan[3];Zhang, Weidong[1,2]

机构：[1]Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China;[2]China State Inst Pharmaceut Ind, Shanghai Inst Pharmaceut Ind, Shanghai 201203, Peoples R China;[3]Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol,Key Lab Synthet Biol, Shanghai 200032, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]Logist Engn Univ, Dept Math, Chongqing 400016, Peoples R China

年份：2017

卷号：7

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000397096800008)】；

基金：The work was supported by Professor of Chang Jiang Scholars Program, NSFC ( 81230090, 81520108030, 81573318, 31271409, 81373301, 1302658, 61372194, 81260672), Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products (10DZ2251300), the Scientific Foundation of Shanghai China ( 12401900801, 13401900103, 13401900101), National Major Project of China (2011ZX09307-002-03), special Fund for strategic pilot technology Chinese Academy of Sciences (XDA08020104) and the National Key Technology R&D Program of China (2012BAI29B06).

语种：英文

摘要：Traditional Chinese medicines (TCMs) have important therapeutic value in long-term clinical practice. However, because TCMs contain diverse ingredients and have complex effects on the human body, the molecular mechanisms of TCMs are poorly understood. In this work, we determined the gene expression profiles of cells in response to TCM components to investigate TCM activities at the molecular and cellular levels. MCF7 cells were separately treated with 102 different molecules from TCMs, and their gene expression profiles were compared with the Connectivity Map (CMAP). To demonstrate the reliability and utility of our approach, we used nitidine chloride (NC) from the root of Zanthoxylum nitidum, a topoisomerase I/II inhibitor and alpha-adrenoreceptor antagonist, as an example to study the molecular function of TCMs using CMAP data as references. We successfully applied this approach to the four ingredients in Danshen and analyzed the synergistic mechanism of TCM components. The results demonstrate that our newly generated TCM data and related methods are valuable in the analysis and discovery of the molecular actions of TCM components. This is the first work to establish gene expression profiles for the study of TCM components and serves as a template for general TCM research.