文献类型：期刊文献

英文题名：A recycled batch biotransformation strategy for 22-hydroxy-23,24-bisnorchol-4-ene-3-one production from high concentration of phytosterols by mycobacterial resting cells

作者：Hu, Yu[1];Wang, Da[1];Wang, Xuedong[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：42

期号：12

起止页码：2589

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20203409066020);WOS:【SCI-EXPANDED(收录号:WOS:000560739200002)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Numbers 31570079, 21276083).

语种：英文

外文关键词：Mycobacterium neoaurum; Phytosterols; Recycled batch biotransformation; Hydroxypropyl-beta-cyclodextrin; 22-Hydroxy-23; 24-bisnorchol-4-ene-3-one

摘要：Objectives To realize a practical technology for recycling both cyclodextrin and resting-cells at the same time in phytosterol biotransformation using mycobacterial resting cells. Results In order to produce 22-hydroxy-23,24-bisnorchol-4-ene-3-one (HBC) efficiently and low-costly, a recycled phytosterols (PS) biotransformation process using mycobacterial resting cells was developed. By optimizing the ratio of hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and PS to 1:1 (w/w), most products crystallized during the biotransformation process. So, the HBC was easily separated by low-speed (900xg) centrifugation with yield of 92%. The resting cells, HP-beta-CD and the residual products and substrates left in the reaction system were reused for another bioconversion cycle after PS supplement. Three continuous cycles were achieved without the supplement of cells and HP-beta-CD. In each batch, 80 g L-1 of PS was transformed to HBC with the space-time yield of HBC of 8.9-12.8 g L(-1)day(-1). Conclusions This strategy reduced the cost of HBC production and simplified the purification process.