文献类型：期刊文献

英文题名：Engineered Bacteria for Visualization and Localization of Gastrointestinal Bleeding: A Promising Application

作者：Zou, Zhen-Ping[1];Wang, Junshan[2];Zhang, Qian[1];Ye, Bang-Ce[1];Zhou, Ying[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Gastroenterol, Shanghai 200072, Peoples R China

年份：2024

卷号：96

期号：34

起止页码：13854

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20243416907332);WOS:【SCI-EXPANDED(收录号:WOS:001291768600001)】；

基金：This work was sponsored by the National Key Research and Development Program of China (2023YFF1204500), the National Natural Science Foundation of China (22134003), and China Postdoctoral Science Foundation (2023M741176).

语种：英文

外文关键词：Gastroenterology

摘要：Gastrointestinal bleeding, especially obscure gastrointestinal bleeding (OGIB), is a common and serious clinical emergency with a notable incidence rate. However, the current diagnostic method, gastroscopy, is invasive and often struggles to efficiently detect microhemorrhagic lesions, leading to diagnostic challenges and potential misdiagnoses. Here, we developed an intelligently engineered bacterium utilizing synthetic biology techniques for in vivo localization detection of gastrointestinal bleeding. By constructing three gene circuit modules within E. coli Nissle 1917 for heme recognition, response, and output generation, we have successfully enabled specific heme sensing and real-time optical signal production in vivo. This innovative strategy overcomes the limitations of the existing diagnostic methods, offering a noninvasive and precise means of detecting gastrointestinal bleeding. These advancements hold promise for enhancing diagnostic accuracy and treatment efficacy in future clinical settings within the realm of gastroenterology.