文献类型：期刊文献

英文题名：Using Chlorella vulgaris to treat toxic excess sludge extract, and identification of its response mechanism by proteomics approach

作者：Wang, Lu[1,2,3,4];Wang, Hualin[1,2];Chen, Xiurong[1,2];Xu, Yan[1,2];Zhou, Tianjun[1,2];Wang, Xiaoxiao[1,2];Lu, Qian[3,4];Ruan, Roger[3,4]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Natl Engn Lab High Concentrat Refractory Organ Wa, Shanghai 200237, Peoples R China;[3]Univ Minnesota, Ctr Biorefining, 1390 Eckles Ave, St Paul, MN 55108 USA;[4]Univ Minnesota, Dept Bioprod & Biosyst Engn, 1390 Eckles Ave, St Paul, MN 55108 USA

年份：2018

卷号：253

起止页码：188

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20180604775866);WOS:【SCI-EXPANDED(收录号:WOS:000426155700024)】；

基金：This work was supported by the Shanghai Pujiang Program in China [Grant No. 13PJD009]; the Natural Science Foundation of China [Grant No. 51378207] and the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2014ZX07202-011-002). The authors also would like to express their appreciation to Chinese Scholarship Council (CSC).

语种：英文

外文关键词：Chlorella vulgaris; Excess sludge; Toxicity removal; Response mechanism; Proteomics analysis

摘要：Chlorella vulgaris was cultivated in varying proportions of toxic sludge extracts obtained from a sequencing batch reactor for treating synthetic wastewater containing chlorophenols. C. vulgaris could reduce the ecotoxicity from sludge extracts, and a positive correlation was noted between ecotoxicity removal and total organic carbon removal. In terms of cell density, the optimal proportion of sludge extracts required for the cultivation of C. vulgaris was lower than 50%. The correlation between protein content in per 10(6) algae and inhibition extent of ecotoxicity of the 5 groups on the day of inoculation (0.9182, p < .05) indicated a positive relationship between algal protein secretion and ecotoxicity. According to the protein expression and differential protein expression analysis, we concluded that C. vulgaris produced proteins that involved in the stress response/redox system and energy metabolism/biosynthesis to respond to the toxic environment and some other proteins related to mixotrophic metabolism.