文献类型：期刊文献

英文题名：A multi-channel responsive AuNP@COF core-shell nanoprobe for simultaneous subcellular profiling of multiple cancer biomarkers

作者：Lv, Jian[1];Chang, Shuai[1];Chen, Hua-Ying[1];Zhou, Xin-Yue[1];Wang, Xiao-Yuan[1];Chen, Zhen-Chi[1];Chen, Bin-Bin[1];Qian, Ruo-Can[1];Li, Da-Wei[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr Fronti, Dynam Chem Sch Chem, Key Lab Adv Mat,Mol Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：234

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20231814047658);WOS:【SCI-EXPANDED(收录号:WOS:001058003600001)】；

基金：This research was supported by National Natural Science Foundation of China (21974046, 21977031, 22176058), the China Postdoctoral Science Foundation (2021M701195), Shanghai Science and Technology Committee (19520744000, 22ZR1416800, 23ZR1416100), the Program of Introducing Talents of Discipline to Universities (B16017), and the Fundamental Research Funds for the Central Universities (222201717003). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Cancer biomarkers; COF; Fluorescence; SERS; NF; & kappa;B pathway

摘要：The abnormal change in the expression profile of multiple cancer biomarkers is closely related to tumor progression and therapeutic effect. Due to their low abundance in living cells and the limitations of existing imaging techniques, simultaneous imaging of multiple cancer biomarkers has remained a significant challenge. Here, we proposed a multi-modal imaging strategy to detect the correlated expression of multiple cancer biomarkers, MUC1, microRNA-21 (miRNA-21) and reactive oxygen (ROS) in living cells, based on a porous covalent organic framework (COF) wrapped gold nanoparticles (AuNPs) core-shell nanoprobe. The nanoprobe is functionalized with Cy5-labeled MUC1 aptamer, a ROS-responsive molecule (2-MHQ), and a miRNA-21-response hairpin DNA tagged by FITC as the reporters for different biomarkers. The target-specific recognition can induce the orthogonal molecular change of these reporters, producing fluorescence and Raman signals for imaging the expression profiles of membrane MUC1 (red fluorescence channel), intracellular miRNA-21 (green fluorescence channel), and intracellular ROS (SERS channel). We further demonstrate the capability of the cooperative expression of these biomarkers, along with the activation of NF-?B pathway. Our research provides a robust platform for imaging multiple cancer biomarkers, with broad potential applications in cancer clinical diagnosis and drug discovery.