文献类型：期刊文献

英文题名：Tuning the sensing range of potassium ions by changing the loop size of G-quadruplex sensors

作者：Wu, Ting[1];Zhang, Chuanjing[1];Wang, Zhenping[1];Ren, Hongxin[1];Kang, Yan[1];Du, Yiping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：40

期号：11

起止页码：9285

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000387621500037)】；

基金：This work was supported by the National Natural Science Foundation of China (21205041) and Science and Technology Commission of Shanghai Municipality (14142201400). The authors also thank Mr Yawei Li and Miss Min Yang of Malvern Instruments Ltd for their help in ITC data analysis.

语种：英文

摘要：Here, the effect of loop size in a series of G-quadruplex ((G(3)T(2)A)(3)G(3), (G(3)T(2))(3)G(3) and (G(3)T)(3)G(3)) sensors on sensing ability for potassium ions (K+) was evaluated. Fluorescence spectroscopy, mass spectrometry, circular dichroism and isothermal titration calorimetry were applied to investigate their different performances. The results demonstrated that the saturation concentration and the response range of K+ changed to higher concentration as the loop size of the G-quadruplex sensor increased. The association constants decreased along with the increase of loop size, hence more potassium ions were needed for the equilibrium which agreed with their different sensing ranges. In brief, varying loop sizes of the G-quadruplex can efficiently tune the stability of the G-quadruplex and the sensing range of potassium ions. This may guide the design of a new G-quadruplex sensor.