文献类型：期刊文献

英文题名：Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ¹-Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene-3,17-Dione or 1,4-Androstadiene-3,17-Dione

作者：Wei, Wei[1];Wang, Feng-qing[1];Fan, Shu-yue[1];Wei, Dong-zhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：76

期号：13

起止页码：4578

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000279082800057)】；

基金：This research was financially supported by the National Basic Research Program of China (no. 2009CB724703), the National High Technology Research and Development Program of China (no. 2008AA02Z209), and the National Special Fund for State Key Laboratory of Bioreactor Engineering, grant no. 2060204.

语种：英文

摘要：3-Ketosteroid-Delta(1)-dehydrogenase, KsdD(M), was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) is a key bottleneck to the microbial transformation of phytosterols in industry. This problem was tackled via genetic manipulation of the KsdD-encoding gene. Mutants in which KsdD(M) was inactivated or augmented proved to be good AD(D)-producing strains.