文献类型：期刊文献

英文题名：Production of interferon alpha A by Escherichia coli W3110 (pEC901).

作者：Kang, F[1];Ye, Q;Yu, J;Zhang, S

机构：[1]Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.

年份：1993

卷号：9

期号：4

外文期刊名：Chinese journal of biotechnology

收录：MEDLINE(收录号：8061237);;PubMed

语种：英文

摘要：The maximum specific growth rate, saturation constant, maintenance coefficient and growth yield for E. coli W3110 (pEC901) and the host, W3110, were estimated through continuous cultivation of the two strains. The growth rate of the transformant was lower than that of the host, while its maintenance metabolism increased due to the existence of plasmids pEC901. Both the plasmid stability and the expression level of interferon increased with the increase in dilution rate. In fed-batch culture, the growth rate of W3110 (pEC901) was maintained at a high level by controlling the feeding rate of glucose, and the interferon titer reached 2.5 x 10(10) u/l, which was greatly improved compared with those reached in shaker flask cultures.