文献类型：期刊文献

英文题名：Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse

作者：Wang, Yuqiu[1,2];Li, Junmin[3];Lu, Dayun[2];Meng, Qian[2];Song, Nixue[2];Zhou, Hu[2];Xiao, Xiao[1];Sun, Liming[4];Zhu, Hongwen[2]

机构：[1]East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China;[3]Fudan Univ, Zhongshan Hosp, Surg Crit Care Med, Xuhui Branch, Shanghai, Peoples R China;[4]Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China

年份：2022

卷号：255

外文期刊名：JOURNAL OF PROTEOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000787266500006)】；

基金：This study is supported by the Yangfan Project of Shanghai Science and Technology Commission (No.20YF1457300). We would like to thank the Institutional Technology Service Center of Shanghai Institute of Materia Medica for all the technical support.

语种：英文

外文关键词：Brown adipose tissue; Subcutaneous white adipose tissue; High fat diet; Proteomics; Phosphoproteomics; Metabolism

摘要：Thermogenesis is a promising approach to limit weight gain in response to excess nutrition. In contrast to cold induced thermogenesis, the molecular and cellular mechanisms of diet-induced thermogenesis (DIT) have not been fully characterized. Here, we explored the response of brown adipose tissue (BAT) and subcutaneous white adipose tissue (sWAT) to high fat diet (HFD) using proteome and phosphoproteome analysis. We observed that after HFD, Uncoupling protein 1 (UCP1) and its phosphorylation were only increased in BAT. Furthermore, proteins involved in fatty acid oxidation, tricarboxylic acid cycle, and oxidative phosphorylation were also upregulated in BAT. Nevertheless, most metabolic related proteins were downregulated in sWAT. We found that these metabolic changes accompanied with different variation of mitochondrial proteins between BAT and sWAT. After HFD, most mitochondrial proteins were decreased in sWAT, but not in BAT. This effect was correlated with decreased mitochondrial ribosomal proteins in sWAT. Finally, through phosphoproteomic analysis, we predicted the activities of kinases in HFD mice and observed that there were more kinases inactivated in sWAT. Finally, this dataset provides a valuable resource for molecular researchers in the fields of obesity and obesity-related disease. Significance: Thermogenesis is a promising approach to combat obesity in response to excess energy. Nevertheless, the molecular and cellular mechanisms of DIT have not been fully characterized. Herein, we employed mass spectrometry (MS)-based proteomics and phosphoproteomics to identify differentially regulated proteins and phosphosites in BAT and sWAT of mice fed with HFD. This study unveils the differential regulatory networks of HFD in BAT and sWAT, which provides reference omics data to future researchers.