文献类型：期刊文献

英文题名：Characterization of anti-BCG benz[α]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomycete Nocardia sp. XJ31

作者：Zhang, Li[1,2,3];Zhang, Jingyu[1];Ren, Biao[4,5];Lu, Wanying[1];Hou, Chengjian[1];Wang, Jian[2];Ma, Xiaolong[2];Ma, Rong[1];Liu, Mei[2];Liu, Zhiheng[2];Li, Jin-Ping[6];Ding, Kan[7,8];Dai, Huanqin[2];Zhang, Lixin[1];Liu, Xueting[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol SKLM, Beijing 100101, Peoples R China;[3]Boston Univ, Dept Chem, 590 Commonwealth Ave, Boston, MA 02215 USA;[4]Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China;[5]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China;[6]Uppsala Univ, Biomed Ctr, Dept Med Biochem & Microbiol, SciLifeLab Uppsala, Husatgatan 3, S-75123 Uppsala, Sweden;[7]Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, Shanghai 201203, Peoples R China;[8]Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China

年份：2020

卷号：104

期号：19

起止页码：8267

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20203509098534);WOS:【SCI-EXPANDED(收录号:WOS:000562287500004)】；

基金：This study was financially supported by the National Key Research and Development Program of China (2019YFA0906200), the National Natural Science Foundation of China (31720103901, 21877038, 21977029, 21877124, 81573341), Shanghai Rising-Star Program (20QA1402800), the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), Shanghai Science and Technology Commission (18JC1411900).

语种：英文

外文关键词：Xinjiang desert; Nocardiasp; Anti-BCG; Brasiliquinone; Nocardimicin

摘要：The current global demand for novel anti-TB drugs has drawn urgent attention on the discovery of natural product compounds with anti-TB activity. Lots of efforts have emphasized on environmental samples from unexplored or underexplored natural habits and identified numerous rare actinomycete taxa producing structurally diverse bioactive natural products. Herein, we report a survey of the rare actinobacteria diversity in Xinjiang region together with the discovery of anti-TB active natural products from these strains. We have collected 17 soil samples at different sites with different environmental conditions, from which 39 rare actinobacteria were identified by using a selective isolation strategy with 5 media variations. Among those isolated strains, XJ31 was identified as a newNocardiasp. based on 16S rRNA gene analysis. Through one strain-many compounds (OSMAC) strategy combined with anti-Bacillus Calmette-Guerin bioassay-guided isolation, two groups of compounds were identified. They were twelve siderophores (nocardimicins,1-12) and two anthraquinones (brasiliquinones,13and14) and ten of them were identified as new compounds. The structures of the purified compounds were elucidated using HR-ESI-MS, 1D NMR, and 2D NMR techniques. The anti-TB bioassays revealed that the two benz[alpha]anthraquinones have potent activity against BCG (MICs = 25 mu M), which can be used as a promising start point for further anti-TB drug development.