文献类型：期刊文献

英文题名：pH Responsiveness of Hexosomes and Cubosomes for Combined Delivery of Brucea javanica Oil and Doxorubicin

作者：Li, Yawen[1,2];Angelova, Angelina[3];Hu, Fangzhou[1,2];Garamus, Vasil M.[4];Peng, Changjun[1,2];Li, Na[5,6];Liu, Jianwen[1,2];Liu, Dan[1,2];Zou, Aihua[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, State Key Lab Bioreactor Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Paris Saclay, Univ Paris Sud, CNRS, UMR 8612,Inst Galien Paris Sud,LabEx LERMIT, F-92296 Chatenay Malabry, France;[4]Helmholtz Zentrum Geesthacht, Ctr Mat & Coastal Res, D-21502 Geesthacht, Germany;[5]Natl Ctr Prot Sci Shanghai, Shanghai 200237, Peoples R China;[6]Shanghai Inst Biochem & Cell Biol, Shanghai 200237, Peoples R China

年份：2019

卷号：35

期号：45

起止页码：14532

外文期刊名：LANGMUIR

收录：;EI(收录号：20194607666825);WOS:【SCI-EXPANDED(收录号:WOS:000497262300016)】；

基金：This work was supported by the National Natural Science Foundation of China (Grants Nos. 21573070, 21872051, and U1832144)

语种：英文

外文关键词：Crystalline materials - X ray scattering - Targeted drug delivery - Controlled drug delivery - Diseases - Emulsification - Drug products - Microemulsions - Cell death - Nanoparticles - Biosynthesis - Cell culture

摘要：We report pH-responsive liquid crystalline lipid nanoparticles, which are dual-loaded by Brucea javanica oil (BJO) and doxorubicin hydrochloride (DOX) and display a pH-induced inverted hexagonal (pH = 7.4) to cubic (pH = 6.8) to emulsified microemulsion (pH = 5.3) phase transition with a therapeutic application in cancer inhibition. BJO is a traditional herbal medicine that strongly inhibits the proliferation and metastasis of various cancers. Doxorubicin is an antitumor drug, which prevents DNA replication and hampers protein synthesis through intercalation between the base pairs of the DNA helices. Its dose-dependent cardiotoxicity imposes the need for safe delivery carriers. Here, pH-induced changes in the structural and interfacial properties of designed multicomponent drug delivery (monoolein-oleic acid-BJO-DOX) systems are determined by synchrotron small-angle X-ray scattering and the Langmuir film balance technique. The nanocarrier assemblies display good physical stability in the studied pH range and adequate particle sizes and zeta-potentials. Their interaction with model lipid membrane interfaces is enhanced under acidic pH conditions, which mimic the microenvironment around tumor cells. In vitro cytotoxicity and apoptosis studies with BJO-DOX dual-loaded pH-switchable liquid crystalline nanoparticles are performed on the human breast cancer Michigan Cancer Foundation-7 (MCF-7) cell line and MCF-7 cells with doxorubicin resistance (MCF-7/DOX), respectively. The obtained pH-sensitive nanomedicines exhibit enhanced antitumor efficacy. The performed preliminary studies suggest a potential reversal of the resistance of the MCF-7/DOX cells to DOX. These results highlight the necessity for further understanding the link between the established pH-dependent drug release profiles of the nanocarriers and the role of their pH-switchable inverted hexagonal, bicontinuous cubic, and emulsified microemulsion inner organizations for therapeutic outcomes.