文献类型：期刊文献

英文题名：Simultaneous detection of 5-fluorocytosine and 5-fluorouracil in human cells carrying CD/5-FC suicide gene system by using capillary zone electrophoresis

作者：Liu, Yajing[1,2];Zhu, Pan[1];Huang, Zhiwei[3];Zhou, Li[1];Shi, Ping[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Organofluorine Chem, Shanghai, Peoples R China;[3]Donghua Univ, Coll Chem Chem Engn & Biotechnol, 2999 Renmin Rd, Shanghai 201620, Peoples R China

年份：2018

卷号：1076

起止页码：1

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20180604752207);WOS:【SCI-EXPANDED(收录号:WOS:000425555300001)】；

基金：This work was sponsored by grants from the National Natural Science Foundation of China (31671309).

语种：英文

外文关键词：CD/5-FC system; Capillary zone electrophoresis; 5-FC; 5-FU; Human cells

摘要：A well-known suicide gene therapy approach, cytosine deaminase (CD) in combination with prodrug 5-flurcytosine (5-FC), has become an effective strategy of tumor treatment. However, there are short of simple and convenient detection methods to evaluate the efficiency of 5-FC conversion to 5-fluorouracil (5-FU) in human cells carrying various CD/5-FC systems. In this study, we developed an effective capillary zone electrophoresis (CZE) method to simultaneously measure 5-FC and 5-FU in cells carrying CD/5-FC suicide gene system. Under the condition of 60 mM borate buffer (pH 9.5) and 25 kV separation voltage with 0.5 psi x 15 s injection in 210 nm, the separation of 5-FC and 5-FU could be completely achieved within 15 min. The linearity of the calibration curve of standard 5-FC and 5-FU was in the range from 1 to 1000 mu M (r(2) > 0.999) and their recoveries were 98.4% and 96.0%, respectively. Due to the simple sample preparation and easy detection, this method is suitable for the study of the conversion efficiency of CD/5-FC suicide gene system. It aims to intuitively evaluate CD/5-FC systems and helps to guide the improvement of more effective CD/5-FC suicide gene systems.