文献类型：期刊文献

英文题名：Efficient Stereoselective Synthesis of Structurally Diverse γ- and δ-Lactones Using an Engineered Carbonyl Reductase

作者：Chen, Meng[1];Zhang, Xiao-Yan[1];Xing, Chen-Guang[2];Zhang, Chao[1];Zheng, Yu-Cong[1];Pan, Jiang[1];Xu, Jian-He[1];Bai, Yun-Peng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Xiamen Oamic Biotech Co Ltd, 36 Longmen Rd, Xiamen 361026, Fujian, Peoples R China

年份：2019

卷号：11

期号：11

起止页码：2600

外文期刊名：CHEMCATCHEM

收录：;EI(收录号：20192006939598);WOS:【SCI-EXPANDED(收录号:WOS:000470937300005)】；

基金：This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300), National Natural Science Foundation of China (Nos. 21536004, 21776085 & 21871085), Natural Science Foundation of Shanghai (18ZR1409900 & 18DZ1112703) and the Fundamental Research Funds for the Central Universities (22221818014, 222201714026 & WF1714026).

语种：英文

外文关键词：Asymmetric reduction; directed evolution; chiral lactones; carbonyl reductase; biocatalysis

摘要：Structurally diverse gamma- and delta-lactones were efficiently synthesized stereoselectively using an engineered carbonyl reductase from Serratia marcescens (SmCRV4). SmCRV4 exhibited improved activity (up to 500-fold) and thermostability toward 14 gamma-/delta-keto acids and esters, compared with the wild-type enzyme, with 110-fold enhancement in catalytic efficiency (k(cat)/K-m) toward methyl 4-oxodecanoate. The preparative synthesis of alkyl and aromatic gamma- and delta-lactones with 95 %->99 % ee and 78 %-90 % yields was demonstrated. The highest space-time yield, 1175 g L-1 d(-1), was achieved for (R)-gamma-decalactone.