文献类型：期刊文献

英文题名：Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways

作者：Liu, Min[1];Lou, Jiali[1];Gu, Jiali[2];Lyu, Xiao-Mei[3];Wang, Feng-Qing[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Huzhou Univ, Coll Life Sci, Huzhou, Peoples R China;[3]Nanyang Technol Univ, Coll Engn, Sch Chem & Biomed Engn, Singapore, Singapore

年份：2020

卷号：314

起止页码：1

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20201508391238);WOS:【SCI-EXPANDED(收录号:WOS:000533862500001)】；

基金：This study was funded by the National Natural Science Foundation of China (No. 31500043), the Natural Science Foundation of Shanghai (No.19ZR1473000), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：L-Homoserine; Central metabolic pathways; Metabolic engineering; Escherichia coli

摘要：L-homoserine is an important functional amino acid. Based on the system metabolic engineering strategy, the key genes in the central metabolic pathway of Escherichia coli W3110 were engineered to construct the strain for Lhomoserine production. To construct an engineered strain with high yield of L-homoserine, the work was carried out from the following aspects: (1) Disrupt the competitive and degradative pathways of L-homoserine, and the Lhomoserine was initially accumulated with a titer of 0.2 g/L; (2) Exploring the effect of weakening TCA cycle, modification of the glyoxylate branch, and reduction of the pyruvate synthesis for L-homoserine synthesis. The concentration of L-homoserine in the final recombinant strain LJL12 reached a titer of 3.2 g/L at shake flask and 35.8 g/L in fed-batch fermentation, showing a high L-homoserine production capacity (0.82 g/L/h). The study provides a well research foundation for L-homoserine production with the capacity for industrial application.