文献类型：期刊文献

英文题名：One-Pot Synthesis of Phenylglyoxylic Acid from Racemic Mandelic Acids via Cascade Biocatalysis

作者：Tang, Cun-Duo[1,2,3];Ding, Peng-Ju[1,2];Shi, Hong-Ling[1,2];Jia, Yuan-yuan[1,2];Zhou, Mao-zhi[3];Yu, Hui-lei[3];Xu, Jian-He[3];Yao, Lun-Guang[1,2];Kan, Yun-Chao[1,2]

机构：[1]Nanyang Normal Univ, Henan Prov Engn Lab Insect Bioreactor, 1638 Wolong Rd, Nanyang 473061, Henan, Peoples R China;[2]Nanyang Normal Univ, Henan Key Lab Ecol Secur Water Source Reg Mid Lin, 1638 Wolong Rd, Nanyang 473061, Henan, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：67

期号：10

起止页码：2946

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20191106637988);WOS:【SCI-EXPANDED(收录号:WOS:000461538100023)】；

基金：This work was supported by the grants from Scientific and Technological Project of China (31870917), Scientific and Technological Project of Henan Province (182102110084 and 162102210116), Scientific Research and Service Platform Fund of Henan Province (2016151), Fund of Scientific and Technological Innovation Team of Water Ecological Security for Water Source Region of Midline of South-to-North Diversion Project of Henan Province (17454), and the Special Funded Projects of Nanyang Normal University (2018QN004).

语种：英文

外文关键词：mandelate dehydrogenase; mandelate racemase; cascade reaction; phenylglyoxylic acid; whole cell catalysis

摘要：Phenylglyoxylic acid (PGA) are key building blocks and widely used to synthesize pharmaceutical intermediates or food additives. However, the existing synthetic methods for PGA generally involve toxic cyanide and complex processes. To explore an alternative method for PGA biosynthesis, we envisaged cascade biocatalysis for the one-pot synthesis of PGA from racemic mandelic acid. A novel mandelate racemase named ArMR showing higher expression level (216.9 U.mL(-1) fermentation liquor) was cloned from Agrobacterium radiobacter and identified, and six recombinant Escherichia coli strains were engineered to coexpress three enzymes of mandelate racemase, D-mandelate dehydrogenase and L-lactate dehydrogenase, and transform racemic mandelic acid to PGA. Among them, the recombinant E. coli TCD 04, engineered to coexpress three enzymes of ArMR, LhDMDH, and LhLDH, can transform racemic mandelic acid (100 mM) to PGA with 98% conversion. Taken together, we provide a green approach for one-pot biosynthesis of PGA from racemic mandelic acid.