文献类型：期刊文献

英文题名：The enantioselective study of the toxicity effects of chiral acetochlor in HepG2 cells

作者：Wang, Susu[1];Zhang, Yang[1];Gao, Jufang[2];Zhang, Jingdi[3];Tao, Liming[1];Xu, Wenping[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life, Shanghai 200234, Peoples R China;[3]Kao Chem Corp Shanghai, Shanghai, Peoples R China

年份：2021

卷号：218

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000652685400002)】；

基金：This work was financially supported by National Key Technology Research Development Program of China (No.2017YFD0200505) and Innovation Program of Shanghai Municipal Education Commission (NO.201701070002E00037) .

语种：英文

外文关键词：Acetochlor; Enantiomers; Cytotoxicity; Apoptosis; HepG2 cells

摘要：Acetochlor is one of the most widely used chiral herbicides in the world, and it is usually produced and used as racemic form (Rac). The potential effects of acetochlor in human body are mainly induced by its residue in agriculture food. The direct target exposed is the liver in human body. However, the potential toxic and mechanism threat to human liver cells caused by chiral acetochlor has been rarely reported. The purpose of this study is to explore the potential mechanism of the toxicity caused by chiral acetochlor in HepG2 cells. The results revealed that acetochlor and its enantiomers could inhibit cell activity and cause DNA damage in HepG2 cells. The toxicity of Rac was higher than that of the two enantiomers, mainly derived from S configuration. The mechanism is through inducing decreased membrane potential (o psi), up-regulated Bax/BcL-2 expression, caused a cascade reaction, activated casepase-3 and casepase-9 and cleaved PARP, which maybe lead to cell death through apoptotic-signaling pathway in the end. These results illuminate that the genotoxic and cytotoxic risks of chiral acetochlor are major coming from S configuration. It provides a theoretical basis for the production of single pesticide to reduce the effects of human health.