文献类型：期刊文献

中文题名：西索米星发酵液的薄层层析生物显影测定

英文题名：Thin layer chromatography combined with bioautographic detection of sisomicin in fermentation broth

作者：陈剑锋[1];郭养浩[2];张元兴[3];罗义发[4];林春红[2];谢涵宾[4]

机构：[1]福州大学药物生物技术与工程研究所,福州,350002；华东理工大学生物反应器国家重点实验室,上海,200237;[2]福州大学药物生物技术与工程研究所,福州,350002;[3]华东理工大学生物反应器国家重点实验室,上海,200237;[4]福州福兴医药有限公司,福州,350011;

年份：2002

卷号：27

期号：4

起止页码：224

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：福建省教育厅科研项目

语种：中文

中文关键词：西索米星;薄层层析;生物显影法;发酵液;抗生素

外文关键词：KEYWORDS: Sisomicin; TLC combined with autobiography assay; Fermentation broth

摘要：通过对检定菌的敏感性,检定培养基、展开剂和发酵液样品等影响因素的考察,建立了一种检测发酵液中西索米星浓度的方法.采用短小芽孢杆菌为生物显影检定菌,双层琼脂扩散法于37℃培养16～18h,控制检定培养基的pH为7.5～8.0,发酵液样品pH为4.5～7.0,展开剂为15%的KH2PO4,点样量为10μl,薄板和菌层接触时间为20min等检定条件,能用于发酵液中西索米星效价的直接测定,其线性回归方程为Y=0.0115X+1.747,r=0.9877.在相同条件下,对536μg/ml的西索米星标准液连续测定6次,测定结果为(531 0±12.7)μg/ml,RSD=3.28%,该法具有较高的实验重复性和准确性,分析稳定性基本符合要求.
ABSTRACT: Based on the optimization of determinant factors such as bioassay medium, plate developingsolution, sampling paper, of which were affecting the accuracy of the potency assay of sisomicin in fermentation broth, adirect transfer blotting method, using methylene blue as staining indicatior to measure the inhibition zone was proposed.The linear regression equation was: Y =0.0115±1. 747; r = 0. 9877. In a comparison experiments with a knowbioassay potency of sisomicin 536μg/ml, the results in 6 sucessively determinations were (531. 0 ± 12. 7 ) μg/ml, RSD= 3. 28%.