文献类型：期刊文献

英文题名：Bioelectrochemical methane production from CO2 by Methanosarcina barkeri via direct and H2-mediated indirect electron transfer

作者：Bai, Yang[1,2];Zhou, Lei[1,2];Irfan, Muhammad[1,2,7];Liang, Tian-Tian[1,2];Cheng, Lei[4];Liu, Yi-Fan[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Sand, Wolfgang[5,6];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,8]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[4]Minist Agr, Biogas Inst, Chengdu 610041, Peoples R China;[5]Donghua Univ, Coll Environm Sci & Engn, Minist Environm Protect, Text Pollut Controlling Engn Ctr, Shanghai 201620, Peoples R China;[6]Univ Duisburg Essen, Biofilm Ctr, Essen, Germany;[7]Univ Engn & Technol, Dept Chem Polymer & Composite Mat Engn, KSK Campus, Lahore 54980, Pakistan;[8]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：210

外文期刊名：ENERGY

收录：;EI(收录号：20203309043814);WOS:【SCI-EXPANDED(收录号:WOS:000606796000007)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 41403066), the Research Foundation of Shanghai (No. 15JC1401400), and the Fundamental Research Funds for the Central Universities of China (No. 222201817017).

语种：英文

外文关键词：Electromethanogenesis; CO2 bioconversion; Electron transfer pathway; Pure methanogen

摘要：Electromethanogenesis is a promising strategy for bioconversion of CO2 to CH4 via direct electron transfer (DET) and/or H-2-mediated indirect electron transfer (IET). However, specific contribution ratios of DET or H-2-mediated IET are unclear. Here, pathway preferences were firstly calculated in the "cage" type cathode colonized by Methanosarcina barkeri. The highest CH4 production rate of 4.4 mmol cm(-2).day(-1) was detected from 36 to 72 h at -1.2 V. The average ratios of H-2-mediated IET were calculated as 59.73 +/- 8.29%, 69.45 +/- 20.75%, 69.55 +/- 18.30% and 82.97 +/- 16.13% at -0.6, -0.8, -1.0 and -1.2 V (vs SHE), respectively. While above -0.4 V, only the DET pathway was detected. The real time polymerase-chain reaction amplification at the transcriptional level of ccdA and frhB showed the similar trend for the pathway preference in CH4 production. Results provide specific ratios and preference of CH4 production via these two pathways, which may be used for parameter reference for industrial applications. (C) 2020 Elsevier Ltd. All rights reserved.