文献类型：期刊文献

英文题名：A novel ionic-bonded cellulose stationary phase for saccharide separation

作者：Sheng, Qianying[1];Ke, Yanxiong[1];Li, Kuiyong[1];Yu, Dongping[2];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

年份：2013

卷号：1291

起止页码：56

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20131816301276);WOS:【SCI-EXPANDED(收录号:WOS:000318745400008)】；

基金：This work was supported by National High Technology Research and Development Program of China (863 Program, 2012AA020203) and National Natural Science of China (Grant No. 21135005 and No. 21105100).

语种：英文

外文关键词：Cellulose-coated stationary phase; Ion interaction; Saccharide separation; Hydrophilic interaction liquid chromatography; High-performance liquid chromatography; MALDI-QIT-TOF-MS

摘要：A hydrophilic interaction liquid chromatography (HILIC) stationary phase of cellulose-coated silica was synthesized as a novel saccharide separation material. The material, prepared with a method based on ionic interaction, was demonstrated to be efficient for immobilization of saccharides on silica supports. The method is more efficient than traditional immobilized saccharide stationary phase synthesis methods. It was evolved from a method using anion exchanger microparticles agglomerated onto macroparticles of cation exchangers to produce anion exchangers. Cationic cellulose, which has a large number of hydroxyl groups, was immobilized on sulfonated silica. The cellulose-coated stationary phase we designed used strong hydrogen bonding between cellulose hydroxyl and carbohydrate compounds for HILIC retention and separation. The stationary phase was successfully used to separate the samples of polar compounds and the complex samples of oligosaccharides, and demonstrated good reproducibility and stability. The material exhibited good separation selectivity for carbohydrates and ability to enrich glycosylated peptides. The method described here is easy to achieve, environmentally safe and innovative than other methods. It also has extensive application possibilities to separate other categories polar compounds. (C) 2013 Elsevier B.V. All rights reserved.