文献类型：期刊文献

中文题名：Arthrobacter globiform is酯酶基因的克隆、表达和酶活性测定

英文题名：Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase

作者：李新月[1];叶蕊芳[1];郑黎[1];郑一涛[1];李晴[1];薛建萍[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海农药研究所,上海200032

年份：2006

卷号：32

期号：8

起止页码：930

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20064110166026);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：酯酶基因;克隆;表达;活性测定

外文关键词：esterase gene ; clone ; expression ; esterase activity

摘要：采用聚合酶链反应(PCR)的方法扩增了A rthrobacter g lobiform is中酯酶编码基因,与载体质粒连接后在大肠杆菌BL 21(DE 3)中进行表达。以包涵体形式表达的蛋白在8 m o l/L尿素作用下溶解,经过透析复性后获得了具有活性的粗蛋白。产物活性实验表明,酯酶表现出较高的催化活性,为菊酯作为杀虫剂的应用奠定了基础。
A novel esterase which stereoselectively hydrolyzes the （＋）-trans stereoisomer of ethyl chrysanthemate to produce （＋）-trans-chrysanthemic acid was discussed. The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction （PCR）. The gene was inserted into a vector and expressed in Escherichia coli BL21 （DE3）. The protein expressed in body was dissolve in 8 mol/L urea before dialysis. The purified crude enzyme show high catalysis activity. It may suggest the possibility that （＋）-trans-chrysanthemate is identified as the insecticide.