文献类型：期刊文献

英文题名：A bacterial ghost vaccine against Aeromonas salmonicida infection in turbot ( Scophthalmus maximus )

作者：Zhou, Jie[1];Yu, Ruofan[1];Ma, Yue[1,3];Wang, Qiyao[1,3];Liu, Qin[1,2,4];Zhang, Yuanxing[2];Liu, Xiaohong[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Lab Aquat Anim Dis MOA, Shanghai 200237, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2024

卷号：151

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;EI(收录号：20240153466);WOS:【SCI-EXPANDED(收录号:WOS:001266895000001)】；

基金：This work was supported by grants from National Key Research and Development Program of China (2023YFC2812103) , National Natural Science Foundation of China (32273168) , and the Fundamental Research Funds for the Central Universities (222201231540) .

语种：英文

外文关键词：Aeromonas salmonicida; Bacterial ghost; Cellular immunity; Turbot; Vaccine

摘要：Aeromonas salmonicida is one of the most prevalent pathogens that causes huge economic losses to aquaculture. Effective vaccination is the first choice for preventing infection. Bacterial ghost (BG), an empty bacterial shell devoid of cytoplasm, is a promising vaccine antigen with distinct advantages. Herein, we established strategies for producing a substantial yield of A. salmonicida ghost (ASG) and investigated the immune-protective properties of it. As a result, 2.84 mg/ml NaOH was discovered to be capable of inducing considerable amounts of ASG. Furthermore, the ASG vaccine elicited adaptive immunity in turbots after rapid activation of innate immunity. Even though formalin-killed cells (FKC) produced a few more antibodies than ASG, ASG ultimately provided a much stronger immune protection effect because it strengthened cellular immunity, with a relative percentage survival (RPS) of 50.1 % compared to FKC. These findings demonstrated that ASG effectively activated cellmediated immunity, which helped get rid of microorganisms inside cells. Therefore, this study presented novel perspectives for future research on furunculosis vaccine products based on ASG as an antigen.