文献类型：期刊文献

英文题名：Development of an mRNA vaccine encoding IHNV glycoprotein protects rainbow trout (Oncorhynchus mykiss) from infection

作者：Wang, Jing[1];Wu, Kaixing[1];Liu, Yu[1];Wang, Shiyao[1];Zhao, Li[1];Zhang, Donghui[1];Liu, Runhui[1];Ren, Yuhong[1];Shao, Shuai[1,2,3];Liu, Qin[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[3]Lab Aquat Anim Dis MOA, Shanghai, Peoples R China

年份：2025

卷号：10

期号：1

外文期刊名：NPJ VACCINES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001534251500002)】；

基金：This work was supported by grants from the National Key Research and Development Program (2023YFD2400702), Qingdao Marine Science and Technology Center (11-04), and Shanghai Agricultural Science and Technology Innovation Program (K2023014).

语种：英文

摘要：mRNA vaccines have demonstrated significant potential in preventing human diseases and controlling livestock infections. However, the application of mRNA vaccines in aquaculture, especially on fish, remains limited. Infectious hematopoietic necrosis virus (IHNV) is an RNA virus that mainly affects rainbow trout (Oncorhynchus mykiss), leading to high mortality rates. In this study, we systematically engineered three UTR-optimized mRNA constructs, exhibiting comparable and sustained in vitro antigen expression. Following encapsulation, the mG1-LNP formulation, incorporating endogenous antigen-specifics, conferred robust relative protection against IHNV challenge, accompanied by enhanced levels of IgM and neutralizing antibodies. Furthermore, dose-response profiling identified 10 mu g/dose as the immunologically optimized regimen, eliciting efficient immunogenicity. Moreover, biodistribution analyses revealed complete mG1-LNP clearance from injection sites and hepatic tissues by 28 dpv, confirming favorable biosafety. Collectively, our work demonstrates the successful development of mRNA-LNP vaccine against infectious IHNV in rainbow trout, providing the first empirical demonstration of mRNA-LNP vaccine efficacy in aquaculture.